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Virus-Free Production and Certification of Tissue-Cultured Garlic Planting Materials
This is a technique for mass-producing virus-free and certified garlic planting materials done through a combination of shoot tip culture, meristem culture, shoot multiplication and in vitro bulblet (G0) formation. Bulbs, which have not undergone cleaning-up by meristem culture can be infected with viruses. Using this technique, planting materials are successfully cleaned particularly of the viruses present in the Philippines: ‘poty’ (OYDV – onion yellow dwarf virus, LYSV – leek yellow streak virus), ‘carla’ (GCLV – garlic common latent virus, SLV – shallot latent virus) and ‘allexi’ (MbFV – miteborne filamentous virus, GarVA – garlic virus A, GarVB – garlic virus B, GarVD – garlic virus D) viruses. Virus indexing is done using a combination of biochemical (ELISA), molecular techniques (RT-PCR) and electron microscopy. Increase in the number of planting materials is achieved with the transplant of Go bulblets to potting media under greenhouse or field conditions to produce G1 bulbs and then planting the G1 bulbs in the field to produce G2 bulbs and so on. In vitro-derived bulbs have higher yield in terms of number and size of cloves compared to normally propagated bulbs. At each generation, tissue-cultured bulbs increase in size with a corresponding increase in the number of cloves per bulb. With this technique, garlic-planting materials can be mass-produced and certified to increase yield in a shorter period of time.
Mass propagation technique
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Public Domain


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