The genus Vigna where the mungbean (Vigna radiata L. Wilczek) belongs is the most recalcitrant and difficult to regenerate from cell and tissue cultures. An efficient and reproducible system of plant regeneration is a prerequisite to useful applications of modern plant biotechnology to support the crop improvement programs. A complete system of producing multiple shoots by shoot organogenesis from cotyledonary node (CN) explants taken from 4-d-old seedlings has been developed for different genotypes of mungbean. The system has shown applicability to 6 other cultivated and 2 semi-wild species of Asiatic Vigna comprising the subgenus Ceratotropis. Being simple, efficient and genotype or species-independent, the technique allows the application of Agrobacterium-mediated transformation in mungbean and other related Vigna species. Moreover, the regeneration system was successfully utilized in the rapid clonal multiplication of elite F1 inter-specific hybrids between V. radiata and V. mungo to ensure genetic advance in wide crosses.