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Medium-Term In Vitro Conservation of Coconut (Cocos nucifera L.) Zygotic Embryos
Germplasm collection and maintenance is an important component of any crop improvement program. Vegetatively propagated crops and some recalcitrant crops including coconut are maintained in the field and are subjected to pests and diseases and natural calamities such as drought and floods. In vitro conservation reduces risks associated with field maintenance and serves as a back up for field conservation. Different culture media manipulations that would reduce growth of coconut embryos in cultures were evaluated for medium term conservation. The in vitro culture responses of tall (Laguna Tall, Tacunan Tall, San Ramon Tall, Tagnanan Tall) and dwarf (Malayan Yellow Dwarf) genotypes to developed minimal growth treatments (i.e., Y3 medium + 20 g/L sucrose + activated charcoal, Y3 medium + 0.3M sorbitol or mannitol, reduced nutrient concentrations) has also been investigated. In all the genotypes tested, percentage germination ranged from 30 to 70%. About 20 to 30% of germinated embryos developed into shoots (with or without roots) or plantlets (with 1-2 expanded leaves and roots) after 12 months of continuous culture (without sub-culture). These treatments can be used for in vitro storage of coconut embryos for a year without transfer.
Mass propagation technique
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Public Domain


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