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Induced Twinning in Dairy Cattle by Artificial Insemination and Embryo Transfer
Twinning is an effective way of increasing the declining number of cattle in the country. Twinning in dairy cattle is done through artificial insemination (AI) and embryo transfer (ET). Holstein Friesans (HF) used as donor and recipient cows are injected intramuscularly with 25 mg Dinoprost tromethamine (PGF2-alpha) to synchronize estrus. After three days, both cows will be in estrus and inseminated with frozen-thawed semen from two bulls, Holstein Friesian-Sahiwal bull for the donor and HF bull for the recipient. Seven days after AI, an embryo at the compacted morula stage will be recovered without surgery by flushing the uterus of the donor with phosphate-buffered saline and 5% fetal calf serum. This can be kept at room temperature and then later transferred without surgery into the uterine horn of a recipient cow using an ET gun.
Agricultural production system
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