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In vitro Propagation of Virus-free Shallot Planting Material
Virus-free shallot planting materials can be produced via in vitro shoot multiplication technique using Murashige and Skoog (MS) medium with naphthalene acetic acid (NAA) and 6-Benzyllaminopurine (BAP), followed by in vitro bulblet (G0) formation and finally, field production of generation 1 (G1) to generation (Gn) bulbs. Using the shoot multiplication scheme, shoots of shallot can be produced continuously for up to 40 cycles or more. Tissue-cultured materials of shallot have been field-tested three times at MMSU, twice at BPI Los Baños and four times at IPB. With this scheme, increase in planting materials can be achieved up to G5 (generation 5). At each generation, tissue-cultured bulbs increase in size. Rate of propagation is higher using tissue-cultured materials compared to normally propagated materials.
Mass propagation technique
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Public Domain


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