Serum biochemistry and ultrasonography can be useful diagnostic tools in evaluating the general health condition of long-tailed macaques in rescue and rehabilitation centers. This study was conducted to determine and correlate the serum biochemistry profile of 24 apparently healthy male and female rescued long-tailed macaques (LTM) with the body weight, crown-rump length, and ultrasonic histogram of liver parenchyma, gallbladder lumen, and renal cortices. This study established the correlations of serum biochemistry profile with ultrasonic histogram of liver, gallbladder, and kidneys and morphometry of rescued LTM.